Manganese-induced hematological alteration in Wistar rats


Milan Chandel, Gyan Chand Jain

Aim: Manganese (Mn)is an essential trace metal that acts as cofactor in many cellular enzymes. The present study was designed to evaluate toxic effects of manganese chloride (MnCl2.4H2O) on marker hematological parameters in rats after subchronic exposure and after 60 days of treatment withdrawal. Materials and Methods: Adult male Wistar rats were randomly divided into five groups. In Group I, the rats were treated with vehicle (0.5 mL distilled water) and served as control. The rats in Group II, III, and IV were exposed to MnCl2 (50, 100, and 150 mg/kg b.wt./day, p.o. respectively)for 120 days. Half of the rats of Group IV were followed by 60 days post-exposure recovery period and served as Group V. Results: The results of the study showed significant dose-dependent decrease in red blood cell (RBC) count, hemoglobin (Hb), hematocrit (Hct) value, platelet count, and significant increase in white blood cell count after MnCl2exposure. Whereas no significant changes were observed in mean corpuscular volume, mean corpuscular hemoglobin (MCH) and MCH concentration (MCHC) after treatment. Scanning electron microscopic study of blood showed a dose-dependent increase in abnormal-shaped RBCs in MnCl2 treated rats. Most of the effects in these parameters were recovered after 60 days of treatment withdrawal. Conclusion: The results of the study reveals that MnCl2 exposure resulted in hematological toxicity in rats and most of the changes in these parameters recovered when Mn exposure was ceased.